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. 2015 Apr 23;96(6):1226–1239. doi: 10.1111/mmi.13002

Figure 5.

figure

Eleven transcription regulators are required for normal biofilm development in vitro by CSLM. The indicated wild type or mutant strains were adhered to silicone substrates for 90 min (labeled ‘Adhered’), unadhered cells were removed and adhered cells developed into biofilms for 8, 24 or 48 h. Biofilms were stained with conconavalin A – Alexa 594 and Syto 13 dyes, then imaged by CSLM. Images are maximum intensity projections of the top and side view. Representative images of at least three replicates are shown. Scale bars are 50 μm. See also Figs S4 and 5.