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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: J Neurochem. 2015 Apr 23;133(6):857–869. doi: 10.1111/jnc.13103

Figure 3. GAT-1 is expressed in neurons and astrocytes and GAT-3 only is present in astrocytes.

Figure 3

Samples were obtained from the spinal dorsal horn of normal control rats. (A) and (B) respectively show staining of GAT-1 and GAT-3 in the spinal dorsal horn, note higher expressions of GAT-1 and GAT-3 at the superficial dorsal horn (Scale bar = 100 μM). (C) Shows that expression of GAT-1 is colocalized with MAP2 (a marker for neuronal cytoskeleton) and GFAP (an astrocyte marker), but not with NeuN (a neuronal cell body marker) or OX42 (a microglia marker) (Scale bar = 20 μM). (D) Shows that GAT-3 is predominantly colocalized with GFAP, but not OX42, MAP2, or NeuN (Scale bar = 20 μM).