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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Clin Immunol. 2015 Apr 1;158(2):127–139. doi: 10.1016/j.clim.2015.03.020

Fig. 6. Jacalin, Peptide M, α-Mon IgA, and SSL7 can be used for affinity purification of rhesus IgA from feces.

Fig. 6

(a) 60ng of IgA affinity purified respectively left to right on jacalin, α- Mon IgA, peptide M, and SSL-7 columns were western blotted with α-monkey IgA-peroxidase. (b) 60ng of IgA affinity purified on Jacalin and α-Mon IgA columns, and 450ng of IgA affinity purified on peptide M and SSL7 columns were run on the same blue native-PAGE, exposed for different times using chemiluminescence, then western blotted with α-monkey IgA-peroxidase. 4-20% Tris-glycine gel used for SDS-PAGE in (a), 4-16% Bis-Tris gel used for blue native- PAGE in (b).