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. Author manuscript; available in PMC: 2016 Jun 9.
Published in final edited form as: Cell Rep. 2015 May 21;11(9):1350–1357. doi: 10.1016/j.celrep.2015.04.052

Figure 2. CRTC2, CREB1 and CBP Bind to the Promoters and Stimulate Transcription of MMR Genes.

Figure 2

(A) ChIP analysis monitoring occupancy of CRTC2, CREB1 and CBP on the promoters of EXO1, MSH6, PMS1 and POLD2 or an irrelevant negative control (NC) DNA region.

(B) ChIP analysis monitoring occupancy of CRTC2, CREB1 and CBP on the promoters of EXO1, MSH6, PMS1 and POLD2 in HeLa cells expressing a NS or CREB1 shRNA.

(C) ChIP analysis monitoring occupancy of CRTC2, CREB1 and CBP on the promoters of EXO1, MSH6, PMS1 and POLD2 in control or CRTC2 KO HeLa cells.

(D,E) qRT-PCR monitoring expression of EXO1, MSH6, PMS1 and POLD2 in control or CRTC2 KO HeLa cells (D) or in HeLa cells expressing a NS, CREB1 or CBP shRNA (E). For all graphs, error bars indicate SD. *P<0.05, **P<0.01.

See also Figure S3.