Fig. 5.

Analysis of 384 loci in 98 individuals collected from Orpheus Island and Magnetic Island. a 48 and 50 individuals from the respective reefs were pooled and quantitatively genotyped using qHRM. The allele frequencies between the reefs were tightly conserved between the two populations, with a Pearson’s r of 0.93 and a slope of 0.97. b We further validated 19 SNPs by genotyping individual members of each pool. The Pearson correlation between known and pooled genotypes for these SNPs is 0.93 with a regression slope of 0.77