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. 2015 Jun 10;10(6):e0127325. doi: 10.1371/journal.pone.0127325

Fig 1. Time course of the distribution of AO-stained apoptotic cells in the irradiated brains of wt and p53–/–embryos.

Fig 1

Wt (A, B, C, D) and p53–/–embryos (E, F, G, H) were irradiated with 10 Gy of gamma rays and stained with AO at 3 h (A, E), 12 h (B, F), 24 h (C, G), and 42 h (D, H) after irradiation. Images of clusters of AO-positive spots at higher magnifications (white arrows in squares in B and F) are shown in boxes (I and J) for detailed views of the rosette-shaped clusters of apoptotic neurons, which were fewer and smaller in the OT of p53–/–embryos. Also shown are a bright-field counterpart image for AO-stained fluorescence images (K) and a schematic diagram illustrating the structure of the embryonic medaka brain at stage 30 (L). CE, cerebellum; OT, optic tectum; TE, telencephalon. Scale bars = 50 μm.