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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Reprod Toxicol. 2014 Dec 25;54:66–75. doi: 10.1016/j.reprotox.2014.12.011

Figure 4. Effects of early life exposure to arsenite on gene expression in the mammary gland of female offspring.

Figure 4

Pregnant female rats were treated with arsenite or ethinyl estradiol as described in Figure 1 and the female offspring were examined.

(a) In utero effects on ERα expression in the prepubertal gland. The amount of ERα mRNA was measured on postnatal days 0, 5, 10, 15, 20 and 25 by qRT-PCR assay and normalized to Arbp mRNA (mean±SEM; n=3–11/group; * p<0.05).

(b) In utero effects on ERα expression in the adult gland. Female offspring were ovariectomized on postnatal day 45. On postnatal day 60, the amount of ERα mRNA was measured by a qRT-PCR assay and normalized to Arbp mRNA. Data are presented as fold difference (mean±SEM; n=4–11/group; * p<0.05).

(c–e) In utero effects on gene expression in the adult gland. Female offspring were ovariectomized on postnatal day 45. On postnatal day 60, the amount of Greb1 (c), amphiregulin (d), and PgR (e) mRNA was measured by a qRT-PCR assay, normalized to Arbp mRNA, and presented as fold difference (mean±SEM; n=4–11/group; * p<0.05).