Fig. 5.
The effect of rhPEPD and rhPEPDG278D on ERBB2-ERBB3 heterodimer interaction. [A] CHO-K1/ERBB2 cells were transfected with human ERBB3 for 24 h and then treated with rhPEPD (5 nM), followed by immunoblotting. [B & C] CHO-K1/ERBB2 cells were transfected with human ERBB3 for 24 h and then treated with solvent, rhPEPD or rhPEPDG278D (each at 5 nM) for 1 h, with or without pretreatment with NRG-1 (20 nM, 15 min). Cell lysates were immunoprecipitated using an ERBB2 antibody or an ERBB3 antibody, followed by immunoblotting. For the immunoblots shown in B, sample loading was adjusted to contain an equal amount of ERBB2.