RIC8 was visualized with RIC8 antibody (red), and cell nuclei were visualized with DAPI (blue). (A-F) Transversal cryosections of ovary with oocytes in different follicular stages starting from primordial follicle to Graafian follicle and (G-I) different regions of oviduct are shown. (H) Higher magnification of the region of ampulla and (i, indicated by white box) isthmus. Abbreviations: A, antrum; Amp, ampulla region of oviduct; Cb, basal layer of cilia; Cc, ciliated cell; Ci, cilia; Co, cumulus oophorus; Cr, corona radiata; Cx, cell cortex, Ec, epithelial cells; Fc, follicular cell; Gc, granulosa cells; Gf, Graafian follicle; GV, germinal vesicle; Ist, isthmus region of oviduct; Lp, lamina propria; Lu, lumen; Pc, peg cell; Pf, primary follicle; Pmf, primordial follicle; Po, primary oocyte; Sf, secondary follicle. Scale bars: 50 μm.