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. 2015 Jun 10;10(6):e0129131. doi: 10.1371/journal.pone.0129131

Fig 2. Dynamic changes in localization of RIC8 in the mouse oocyte during meiosis.

Fig 2

RIC8 was visualized with RIC8 antibody (red), microtubules with antibody to β–tubulin (Tub, white), chromatin with DAPI (blue) and F-actin with phalloidin (Ph, green). (A, B) GV- stage oocyte; (C, D) GVBD (germinal vesicle breakdown) stage oocytes; (E-H) oocytes undergoing meiosis I or (I-L) meiosis II. (a`, b`, c`) Higher magnification of the region of chromatin. Yellow arrowheads indicate the RIC8 foci at nucleolus and on chromatin. White arrows point to the meiotic spindle. (D) Negative control. Abbreviations: AnaI, anaphase of meiosis I; DON, disappearance of the nucleolus; GV, germinal vesicle; FR, full rim stage; MetI/MetII, metaphase of meiosis I or II; ms, meiotic spindle; PR, partial rim stage; TelII, telophase of meiosis II. Scale bar: 20 μm.