Fig 5. RIC8 protein expression pattern compared to NuMa or Gαi1/2 proteins during mouse oocyte maturation.
(A-F) Mouse oocytes at meiosis I were double-labeled with RIC8 antibody (green) and NuMA or Gαi1/2 antibodies (red). DNA was stained with DAPI (blue). The overlapping regions of RIC8 and NuMA or Gαi1/2 (yellow to orange) at meiotic spindle indicated with white arrows. White arrowheads indicated cell cortex regions, where RIC8 and Gαi1/2 co-localize. Abbreviations: Ana/Tel I; anaphase/telophase of meiosis I; ms, meiotic spindle; pb, polar body. Scale bar: 10 μm.