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. 2015 Jun 10;10(6):e0129131. doi: 10.1371/journal.pone.0129131

Fig 8. RIC8 downregulation interferes with localization of Gαi1/2 protein to the oocyte cortex.

Fig 8

(A, B) RIC8 protein (green) expression in mouse oocytes was downregulated by microinjection of Ric8 siRNA. DNA was stained with DAPI (blue). (C) Downregulation of Ric8 mRNA expression with siRNA in microinjected oocytes quantified by qRT-PCR. (D) The relative intensity of RIC8 immunofluorescence signal measured by AutoQuant X3. (E, F) Localization of Gαi1/2 (red) in mouse oocytes treated with Ric8 and control siRNA at meiosis I. DNA was stained with DAPI (blue). (G) The relative intensity of Gαi1/2 immunofluorescence signal measured by AutoQuant X3. Abbreviations: Cx, cortex. *P ˂ 0.05, **P ˂ 0.01; Students t test. Error Bars represent ±SEM scores. Scale bar: 10 μm.