. Author manuscript; available in PMC: 2016 Jul 1.

Published in final edited form as: DNA Repair (Amst). 2015 Apr 25;31:41–51. doi: 10.1016/j.dnarep.2015.04.006

Table 1.

Total and specific mutation rates in six yeast strains.

		WT			msh6Δ			pol2-exo^-			pol2-exo^- msh6Δ			pol3-exo^-			pol3-exo^- msh6Δ
Mutation rate (×10⁻⁷)		0.17			3.3			4.2			290			7.7			1300
95% Confidence intervals		(0.16–0.18)			(3.2–3.5)			(3.6–4.8)			(230–260)			(7.3–8.0)			(1000–1600)
# 5FOA Resistant clones sequenced		211			691			170			178			123			361
			Mutation rates (×10⁻⁹)			Mutation rates (×10⁻⁹)			Mutation rates (×10⁻⁹)			Mutation rates (×10⁻⁹)			Mutation rates (×10⁻⁹)			Mutation rates (×10⁻⁹)
	Target size^a	Total	Mutation type^b	Per base^c	Total	Mutation type^b	Per base^c	Total	Mutation type^b	Per base^c	Total	Mutation type^b	Per base^c	Total	Mutation type^b	Per base^c	Total	Mutation type^b	Per base^c
Base substitutions	285	122	9.8	0.034	292	140	0.49	139	340	1.2	137	22000	78	91	570	2	399	140000	500
Transitions	130	47	3.8	0.029	182	87	0.67	20	49	0.38	39	6400	49	64	400	3.1	363	130000	1000
A to G	18	4	0.32	0.018	2	0.96	0.053	1	2.5	0.14	2	330	18	0	*6.3*	*0.35*	2	720	40
T to C	27	7	0.56	0.021	20	9.6	0.35	3	7.4	0.27	2	330	12	37	230	8.6	154	55000	2100
C to T	36	11	0.89	0.025	6	2.9	0.08	4	9.9	0.27	7	1100	32	6	38	1	5	1800	50
G to A	49	25	2	0.041	154	74	1.5	12	30	0.61	28	4600	93	21	130	2.7	202	73000	1500
Transversions	225	75	6	0.027	110	53	0.23	119	290	1.3	98	16000	71	27	170	0.75	36	13000	58
A to T	39	9	0.73	0.019	3	1.4	0.037	91	220	5.8	8	1300	33	5	31	0.8	3	1100	28
T to A	51	7	0.56	0.011	3	1.4	0.028	8	20	0.39	5	810	16	3	19	0.37	3	1100	21
T to G	42	8	0.64	0.015	3	1.4	0.034	0	*2.5*^d	*0.059*	0	*160*	*3.9*	11	69	1.6	11	4000	94
A to C	25	5	0.4	0.016	6	2.9	0.11	6	15	0.59	2	330	13	0	*6.3*	*0.25*	0	*360*	14
C to A	38	11	0.89	0.023	48	23	0.6	1	2.5	0.065	1	160	4.3	5	31	0.82	16	5800	150
G to T	58	19	1.5	0.026	40	19	0.33	11	27	0.47	82	13000	230	2	13	0.22	2	720	12
C to G	20	6	0.48	0.024	1	0.48	0.024	1	2.5	0.12	0	*160*	*8.1*	0	*6.3*	*0.31*	0	*360*	18
G to C	33	10	0.81	0.024	6	2.9	0.087	1	2.5	0.075	0	*160*	*4.9*	1	6.3	0.19	1	360	11

Number of base pairs in URA3 where this substitution confers 5-FOA resistance, from Supplementary Fig. 1.

Rates are the total mutation rate times the proportion of the observed mutations of that type (left-most column) among the total 5-FOA^R clones sequenced.

Mutation rate per phenotypically detectable base pair.

Italicized values in bold are “less than or equal to” rates for events not seen, calculated based on the maximum mutation rate had one event been observed.