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. 2015 May 9;12:15. doi: 10.1186/s12986-015-0011-8

Figure 1.

Figure 1

Tobacco smoke exposure decreases secretion of ADPN while inducing its intracellular accumulation. Panels A,B: Representative immunoblots (A) and summary statistics (B) for dose-dependent effects of TSE on ADPN accumulation in conditioned medium and in cellular homogenates of 3T3-L1 adipocytes during a 20-h incubation. Panels C,D: Representative immunoblots (C) and summary statistics (D) of the time course of the effects of TSE on ADPN accumulation in conditioned medium and in cellular homogenates of 3T3-L1 adipocytes exposed to 1.5% TSE for 0-20 h. Panel E: ADPN concentrations measured by ELISA in the culture supernatants of 3T3-L1 adipocytes exposed for 20 h to 0 (control) or 1.5% TSE . Panel F: Immunoblots of ADPN in conditioned medium and in cellular homogenates of primary mouse adipocytes treated without or with 1.5% TSE for 20 h. Panel G: Immunoblots of total plasma ADPN in mice after RMPI (Control) or TSE injections. Panels B, D, E, G, n = 3-5. In panels B and D, P < 0.01 by ANOVA of all cellular values, and P < 0.01 by ANOVA of all medium values. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control values (0% TSE or t = 0) by the SNK test. In panels E and G, Student’s t-test was used.