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. Author manuscript; available in PMC: 2015 Nov 8.
Published in final edited form as: Science. 2015 May 8;348(6235):711–714. doi: 10.1126/science.aaa3526

Fig. 1.

Fig. 1

Pooled shRNA screen for genes that regulate terminal erythroid differentiation. (A) Schematic of pooled shRNA screen. CD34+ HSC were induced toward erythroid development, transduced with shRNA library on Day 6 and selected with puromycin. On day 19, shRNA proviruses in orthochromatic erythroblasts were quantified by Illumina sequencing. (B) Change in relative abundance of 5530 shRNAs in erythrocyte proteome library after 19 days of differentiation. (C) RNAi Gene Enrichment Ranking (RIGER) analysis of 116 candidate genes based on magnitude of erythropoiesis phenotype. NES= Normalized Enrichment Score. (D) Predicted localization of library components, with percent of genes that influence erythropoiesis in red.