Fig 1. Multiple sequence alignment of the predicted Radopholus similis Rs-CRT protein with other nematode CRT proteins.

GenBank accession numbers: R. similis (AFK76483), Meloidogyne incognita (AAL40720), Caenorhabditis elegans (NP_504575), Necator americanus (CAA07254), Ditylenchus destructor (ACV33082), Bursaphelenchus xylophilus (ADD82420), Pratylenchus goodeyi (AIW66697). Box, the putative signal peptide sequences of Rs-CRT; I-III, the conserved regions characteristic of the calreticulin class; black circle, possible Ca²⁺-binding signal motif one; black triangle, possible Ca²⁺-binding signal motif two; black pentagram, C-terminal endoplasmic reticulum (ER) retention signal sequences; (*), highly conserved amino acid residues; (:), conserved amino acid residues; (.) conserved cysteine residues.