Table 1.

Inter-group: p-values were calculated from Student’s t-tests performed between the short and long isoflurane groups at each time point for each functional metric. The values highlighted in bold represent statistically significant differences between the groups that passed multiple-comparisons testing (SGoF) which indicated significance at p < 0.0319. Significant differences between the groups were found in functional connectivity, maximum spectral power, low-band, high-band, and broad-band power, and global connectivity (see Figures 3 and 4). Interestingly, no significant differences were found after the 2.75 h time point, suggesting the influences of the long isoflurane duration prior to functional imaging had dissipated by the 3.25 h time point

Time point (h)	0.75	1.25	1.75	2.25	2.75	3.25	3.75	4.25	4.75	5.25	5.75
Connectivity	0.019	0.283	0.245	0.746	0.976	0.874	0.992	0.685	0.250	0.110	0.178
Spectral CoM	0.035	0.113	0.490	0.353	0.682	0.132	0.666	0.383	0.365	0.370	0.420
Max. power	0.045	0.008	0.0006	0.079	0.010	0.114	0.572	0.348	0.044	0.412	0.185
Max. location	0.276	0.614	0.250	0.504	0.698	0.042	0.988	0.092	0.246	0.699	0.756
Low-band power	0.048	0.0002	0.004	0.078	0.002	0.771	0.691	0.474	0.359	0.319	0.617
High-band power	0.112	0.005	0.013	0.050	0.016	0.145	0.503	0.098	0.172	0.222	0.534
Broadband power	0.052	0.0001	0.0001	0.0138	0.002	0.149	0.414	0.035	0.123	0.151	0.310
Global connectivity	0.032	0.041	0.029	0.051	0.066	0.159	0.066	0.66	0.114	0.141	0.840