Table 1.
Strains and key plasmids used in this study
| Strains | ||
|---|---|---|
| Strain | Relevant genotype | Source or Reference |
| ES114 | Wild-type V. fischeri | (Boettcher & Ruby, 1990) |
| KV3246 | attTn7::PsypA-lacZ | (Morris & Visick, 2013) |
| KV4389 | attTn7::ermR | (Morris et al., 2011) |
| KV5367 | ΔsypF | This study |
| KV6351 | ΔrscS ΔsypF | This study |
| KV6526 | ΔsypF attTn7::sypG*-FLAG | This study |
| KV6527 | attTn7::sypG*-FLAG | This study |
| KV6586 | ΔsypE ΔsypF attTn7::sypG*-FLAG | This study |
| KV6659 | ΔsypF attTn7::sypF-FLAG | This study |
| KV6692 | ΔsypF attTn7::sypFD549A-FLAG | This study |
| KV6896 | ΔsypF attTn7::sypFH250Q-FLAG | This study |
| KV6921 | ΔsypF attTn7::ermR | This study |
| KV7085 | ΔsypF attTn7::sypFH705Q-FLAG | This study |
| KV7230 | attTn7::sypG*-FLAG PsypA-lacZ | This study |
| KV7231 | ΔsypF attTn7::sypG*-FLAG PsypA-lacZ | This study |
| KV7154 | ΔsypF attTn7::sypFH705Q D549A-FLAG | This study |
| KV7155 | ΔsypF attTn7::sypFH705Q D549A H705Q-FLAG | This study |
| KV7226 | ΔsypF attTn7::sypF-HPt-FLAG | This study |
| KV7371 | IG (yeiR-glmS)::PsypA-lacZ 1 | This study |
| KV7372 | ΔsypF IG (yeiR-glmS)::PsypA-lacZ 1 | This study |
| KV7377 | ΔsypF IG (yeiR-glmS)::PsypA-lacZ attTn7::sypF- HPt-FLAG1 | This study |
| KV7386 | ΔsypF IG (yeiR-glmS)::PsypA-lacZ attTn7::sypF- FLAG1 | This study |
| KV7387 | ΔsypF IG (yeiR-glmS)::PsypA-lacZ attTn7::sypFH705Q-FLAG1 | This study |
| KV7410 | IG (yeiR-glmS)::PsypA-lacZ attTn7::ermR 1 | This study |
| KV7412 | ΔsypF IG (yeiR-glmS)::PsypA-lacZ attTn7::ermR 1 | This study |
| KV7413 | ΔsypF IG (yeiR-glmS)::PsypA-lacZ attTn7::sypF- HPtH705Q-FLAG 1 | This study |
| KV7485 | ΔsypF attTn7::sypF-HPtH705Q-FLAG | This study |
| KV7651 | ΔsypF ΔrscS attTn7::rscS-sypF chimera | This study |
| KV7654 | ΔsypF ΔrscS attTn7::rscS | This study |
| KV7656 | ΔsypF ΔrscS attTn7::sypF | This study |
| KV7657 | ΔsypF ΔrscS attTn7::ermR | This study |
| Key Plasmids | ||
|---|---|---|
| Plasmid | Description | Source or Reference |
| pANN61 | pKV69 + sypF*D549A generated using primer 1295 | This study |
| pANN62 | pKV69 + sypF*H705Q generated using primer 1569 | This study |
| pANN69 | pCLD292 + rscS-sypF chimera-FLAG generated using primers 1899, 1900, 1901, 1882 | This study |
| pARM7 | pKV282 + rscS | (Morris et al., 2011) |
| pCLD29 | pKV69 + sypF* | (Darnell et al., 2008) |
| pCLD54 | pKV69 + sypF | (Darnell et al., 2008) |
| pKV69 | Vector; CmR, TetR | (Visick & Skoufos, 2001) |
| pKV282 | Vector, TetR | (Morris et al., 2011) |
1
IG (yeiR-glmS): intergenic (IG) region between the yeiR and glmS genes directly upstream of the Tn7 site in the chromosome
2
the original sypF* sequence was removed from pCLD29 using restriction enzymes before the insertion of indicated DNA sequences