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. Author manuscript; available in PMC: 2015 Jun 14.
Published in final edited form as: Nature. 2010 Nov 10;468(7324):696–700. doi: 10.1038/nature09536

Figure 2. Oxidant stress is elevated in SNc dopaminergic neurons from DJ-1 knockout mice.

Figure 2

(a) Somatic whole cell recording from a SNc dopaminergic neuron in a brain slice from a DJ-1 knockout mouse, showing normal pacemaking (top) and intracellular calcium oscillations (bottom); similar results were obtained in all five neurons examined. (b) Mitochondrial mito-roGFP oxidation was higher (red trace) than in control SNc dopaminergic neurons (black trace); isradipine pretreatment normalized oxidation of mito-roGFP (green trace); experiments were done at 20-22 °C. (c) Box plot summarizing mean mito-roGFP measurements in wild-type SNc neurons (n=9), DJ-1 knockout SNc neurons (n=6) and DJ-1 knockout neurons after isradipine pretreatment (n=7); differences between wild-type and DJ-1 knockout were significant (P<0.05), as were differences between knockouts with and without isradipine treatment (P<0.05). (d) Box plot summarizing mean mito-roGFP measurements from wild-type VTA dopaminergic neurons (n=9), wild-type SNc dopaminergic neurons (n=14) and DJ-1 knockout VTA dopaminergic neurons (red box) (n=4) at 34-35 °C. VTA dopaminergic neurons were unaffected by DJ-1 deletion (P>0.05).