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. Author manuscript; available in PMC: 2015 Jun 14.
Published in final edited form as: J Immunol Methods. 2008 Nov 11;340(2):116–122. doi: 10.1016/j.jim.2008.10.010

Fig. 2.

Fig. 2

Cardiac perfusion maintains tissue integrity. We measured tissue integrity by comparing the size of nuclei following fixation to that from fresh, unfixed tissue. Nuclei were visualized using DAPI staining and then measured. The relative size of individual nuclei from fresh, unfixed, PFA immersion-fixed and PFA perfusion-fixed tissues are plotted. Immersion fixation led to a decrease in the nuclei sizes by 50% while cardiac perfusion fixation decreased nuclei size only modestly (14%). A total of 2 slides and 5 microscopic fields per slide were analyzed from two mice per fixation method. Error bars denote the mean±SD.