Table 3. Primers to detect polymorphism among P. ginseng accessionsPrimer ID.
Primer sequence (5’- 3’) | Product size (bp) | Location | ||
---|---|---|---|---|
SNP based dCAPS | pgcpd01 a | F: AAATATGACCAACAGTAGTTCGAATCTA | 212/190 | rpoC1 |
R: AGCTTATCGGCAGAAACGAA | ||||
pgcpd02 b | F: ATTTCGGGGACTCACAGAAGTAC | 200/177 | rpoC2 | |
R: AAAGCAATTTACGCGAAGGA | ||||
InDel based markers | pgcp139f*r2 | F: TGTGCGACAAACAAATAAGTCA | 157/150 | rpl32 ~ trnUAG |
R2: CGAAGCGAGTTCCATTTCAT | ||||
pgycf1 | F: GGTATTAGTCTGGATACGGCAAA | 729/672/615 c | ycf1 | |
R: TCGAAAAGAAGGGTCACAAGA | ||||
pgcp097f2*r | F: TGGAAAGGCTGTTGTCACTG | 390/377/344 | rps16 ~ trnUUG | |
R: TCAGCAACGGGAGATATTCA | ||||
pgcp137 | F: TCCTGAACCACTAGACGATGG | 514/455 | trnUUC ~ trnGGU | |
R: TTTCGATAACTTCTTGATCCCTCT |
a, b pdcpd01 and pgcpd02 are dCAPS primer pairs with XbaI and ScaI restriction sites, respectively.
c PCR product size is derived from P. quinquefolius.