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. 2015 May 31;2015:976068. doi: 10.1155/2015/976068

Figure 3.

Figure 3

ATR reduced the amount of AO-positive cells in neural tube of 20 hpf embryos, and resulted in the number of RB cells increased in dorsal spinal cord of 72 hpf larvae. Control embryonic neural tube concentrated large apoptotic cells, green colored point is AO+ cells, also located in the superficial skin (a1), while showing a small amount RB cells (a2). After treatment of ATR (b 0.001 mg/L, c 0.01 mg/L, d 0.1 mg/L), AO+ cells in embryonic neural tube became less (b1, c1, d1); The number of RB neurons increases under the phase contrast microscope (b2, c2, d2). The white arrows indicate the apoptotic RB cells; the blue arrow indicated the survival RB cells. In all photos, juvenile dorsal was to up and the mouth to the right. Scale: (a1, b1, c1, d1) in 100 μm; (a2, b2, c2, d2) of 25 μm. 10 samples were used for each experimental batch. 3–5 batches were reduplicated.