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. 2015 Jun 10;10(6):e0129010. doi: 10.1371/journal.pone.0129010

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Fig 1 — Data are averages of biological triplicates ± SD normalized to CONS7 mRNA. Independent-Samples T Test was used to test the significance. Asterisks indicate significant differences in relative expression between transgenic lines and control. (*, p < 0.05; **, p < 0.01). S24-4D, S-24-13, S-24-15 are T₅ homozygous for the pMUFAD transgene. Soybean cultivar Jack is used as wild-type control. (A) to (C) Normalized gene expression level of GmFAD3A, GmFAD3B and GmFAD3C, respectively. The values of wild-type plants were arbitrarily fixed to 1.0. (D) Percentage of mismatches between the 318-bp IR and corresponding regions in GmFAD3A, GmFAD3B and GmFAD3C, respectively.