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. Author manuscript; available in PMC: 2015 Jun 18.
Published in final edited form as: Nature. 2014 Oct 12;516(7531):432–435. doi: 10.1038/nature13833

Figure 2. Cohesin is required for globule formation.

Figure 2

a, Hi-C heatmap at 10kb resolution for rad21-K1. b, 4C-like profiles showing the average contact probabilities of centromeres and telomeres WT, wild type. c, Distribution of intra-arm, inter-arm and inter-chromosomal contact frequencies. d, Hi-C heatmaps of a segment of chromosome 2 overlaid with blue lines corresponding to cohesin (Psc3) peaks from the 10kb binned profile (top); Hi-C directional preference profile (below). To compare results from different experiments, color scales were chosen such that the maximum value corresponds to the ninety-ninth percentile of intra-arm contact frequencies. The boundaries detected in rad21-K1 may result from random fluctuations in directionality due to experimental limitations, or remaining cooperative factors required for boundary establishment. e, Relative contact probability around a cohesin peak as a function of insulation distance averaged over all cohesin peaks (insulation plot). Depletion of contact probability (blue stripe) is not observed in rad21-K1.