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. Author manuscript; available in PMC: 2015 Jun 18.
Published in final edited form as: Nature. 2014 Oct 12;516(7531):432–435. doi: 10.1038/nature13833

Figure 3. Globules are a feature of chromosome architecture in G1 cells.

Figure 3

a, FACS of cells used for cohesin ChIP and Hi-C. b, Cohesin (Psc3) ChIP enrichment at convergent genes within a region of chromosome 2. c, Psc3 enrichment at convergent genes in wild-type and G1 cells. All pairs of convergent genes were aligned at the 3’ end of the second gene (green box). d, Hi-C heatmaps of a segment of chromosome 2 overlaid with lines corresponding to cohesin peaks from the 10-kb binned profile. Plotted below is the directional preference profile. e, Insulation plot around cohesin peak in G1-arrested cells. f, Hypothetical model showing cohesin bound between locally compacted globule regions. Cohesin confines interactions within individual globule domains and prevents interactions across the boundaries.