Figure 4. Loss of heterochromatin affects genome organization.

a, Hi-C heatmap for clr4Δ. b, 4C-like interaction profiles showing the average contact probabilities of centromeres and telomeres. Inter- and intra-chromosomal interaction profiles are shown for mat. c, Distribution of indicated contact probabilities. d, Hi-C heatmaps of a segment of chromosome 2 overlaid with lines corresponding to cohesin peaks. Directional preference profiles are plotted below. e, Insulation plot around cohesin peak in clr4Δ. f, The cross-like pattern of interactions between centromere-proximal regions of different chromosomes. g, Box and whisker plot showing the distribution of spatial distances between lacO inserted outside a pericentromeric heterochromatin domain and the centromere core marked by either SPB (left) or Cnp3 (right). *P ≤ 0.05, **P ≤ 0.01 (n=90, two-sided Mann-Whitney U test). Scale bars, 1 μm. h, Intra-arm Pc(s) for regions colored according to their distance from the centromere. In wild type, Pc(s) for regions near the centromere decreases more rapidly than for more distal regions. In clr4Δ, decay of Pc(s) for regions near the centromere is similar to that of more distal regions, indicating that the organization and conformations of pericentromeric chromatin are more similar to the organization of other chromosomal regions.