Skip to main content
. Author manuscript; available in PMC: 2015 Jun 14.
Published in final edited form as: J Immunol. 2008 Feb 1;180(3):1432–1441. doi: 10.4049/jimmunol.180.3.1432

FIGURE 4.

FIGURE 4

V(D)J rearrangements across major functional NK subsets. a, Splenic (SPL) CD127+ NK cells in the periphery were resolved within the CD122+NK1.1+ subset lacking lineage (lin) markers (CD3−CD4−CD8−CD19−Gr-1−) and subsequently examined for VEX expression. Expression of CD127 on splenic T cells (TCRβ+) is shown for comparison. b, VEX expression was examined as a function of CD27 and Mac-1 expression in spleen NK cells. The data are representative of two to three independent experiments. FSC, Forward scatter. c, Human peripheral blood NK cells enriched by MACS depletion were subject to two rounds of cell sorting for CD56dimCD3− or CD56bright CD3− NK cells. Vδ2-Dδ3 rearrangements were analyzed by bulk PCR (top panel) or single cell PCR (bottom panel) as described in Materials and Methods. The NALM-16-positive control cell line occasionally amplifies as a doublet for unclear reasons. Data from three independent donors is shown (donor 1, LV68681; donor 2, LV70195; donor 3 KJ91815).