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. Author manuscript; available in PMC: 2015 Jun 14.
Published in final edited form as: J Autoimmun. 2012 Nov 5;42:19–28. doi: 10.1016/j.jaut.2012.10.002

Figure 4.

Figure 4

Autoreactive CD8+ T lymphocytes escape from clonal deletion and accumulate in the liver of TNP5/TTR-NP mice. (A) Intrahepatic lymphocytes from TNP5, TNP5/TTR-NP and control TTR-NP mice were isolated, and the percentage of CD4+ T cells, CD8+ T cells, NP specific CD8+ tetramer-bound T cells was determined by flow cytometry (mean ± SD of 2 independent experiments, n=3–4 mice per group) *p<0.05 and **p<0.001. (B) Total number of intrahepatic T cells in indicated mice was determined by cell counting. The total cell numbers per liver are presented as means ± SD from 3–4 livers for each group. *p<0.05. (C) Gated intrahepatic CD4+ T cells from DTg TNP5/TTR-NP and control mice (TTR-NP) were analyzed for CD25 and intracellular Foxp3 expression (left). The frequency of CD4+ T cells that are FoxP3+ and CD25+ in liver of DTg TNP5/TTR-NP mice was not significantly different compared with that of STg TTR-NP (right, p=.330) (mean ± SD representative of 2 independent experiments, n=3–4 mice per group).