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The change in the number of protein-DNA contacts is shown during the unbinding simulations while pulling the POUS (A,B) or the POUHD (C-F). (C,D) Globular region of the POUHD. (E,F) N-terminal tail of the POUHD. The contacts were divided into recurrent (A,C,E) and non-stable (B,D,F). The fraction of recurrent contacts was calculated using the number of recurrent contacts from the unbiased simulations as reference.
