Fig 1. Expression of clock genes assayed in barley seedlings grown in different light regimes.

Clock gene expression assayed by quantitative reverse transcriptase PCR (qRT-PCR) in 5 day old barley seedlings (cv. Sonja) germinated and grown in constant temperature conditions (20°C) with (A) 12 hour day-night cycles with light from hours 0 to 12, (B) constant darkness or (C) constant light. Average expression in RNA extracted from individual seedlings (3 biological repeats) is shown relative to ACTIN (Rel. exp.), error bars show standard error. Horizontal axis labels indicate the time (hours) relative to when the first sample was harvested. Samples were harvested directly from the described conditions (i.e. without free running conditions for the day-night cycle samples).