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. 2015 Jun 11;10(6):e0128974. doi: 10.1371/journal.pone.0128974

Fig 2. Constitutively active Rho rescues actin stress fibers and paxillin in the presence of apoE3.

Fig 2

(A) VSMCs infected with adenoviruses encoding LacZ or RhoV14 were incubated in 10% FBS with or without apoE3 for 24 hr. Cells were co-stained with phalloidin and anti-paxillin. Scale bar = 50 μm. The zooms show magnified views of paxillin staining in the boxed areas. The average phalloidin (B) and paxillin (C) intensity of single cells was analyzed using ImageJ and normalized to control cells. n = 3 independent experiments with at least 8 cells analyzed per experiment. Data information: Graphs show mean + SEM. *p<0.05 or **p<0.01.