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. 2015 Jun 11;10(6):e0129485. doi: 10.1371/journal.pone.0129485

Fig 3. Pro-inflammatory cytokines profile in lupus nephritis and their effect on DNaseI expression in tubular cells.

Fig 3

Renal mRNA expression of pro-inflammatory cytokines in (NZBxNZW)F1 mice during development of lupus nephritis demonstrated in A. The data demonstrate significant up-regulation of TNFα and IL-1β in Group 2 and Group 3 mice when compared to Group 1. IL-10 mRNA levels were significantly increased in Group 3 BW mice compared to Group 1. One-way ANOVA with Dunett post hoc test was performed to assess the difference in gene expression between the groups when compared to group 1 and 2. Effect of pro-inflammatory cytokines and hypoxia on DNaseI and Trap 1 mRNA levels in human renal proximal tubule epithelial cells (RPTEC) are shown in B. Significant elevation of DNaseI mRNA levels in RPTEC was found only upon TNFα stimulation and stress by hypoxia (B). No change in DNaseI gene expression was observed when RPTEC were stimulated by IL-1β, IFNγ, IL-6 or IL-10 (B). Trap 1 mRNA was significantly reduced after stimulations with IL-1β and hypoxia. Significances: *<0.05; **<0.005; ***<0.0005.