Table 3. Mass spectrometry analysis of renal tubular cell lysates size-fractionated by SDS gel electrophoresis.
Gel regions as MW spans that are subjected to mass spectrometry | Bands recognized by anti-DNaseI antibody on western blot | Presence of DNaseI protein verified by mass spectrometry | Common DNaseI peptides identified by mass spectrometry | |||
---|---|---|---|---|---|---|
Nuclear extract | Cytoplasmic extract | Nuclear extract | Cytoplasmic extract | Nuclear extract | Cytoplasmic extract | |
38–40 kDa | No bands | 40 kDa | No | Yes | Not detected | LLDNLNQDAPDTYHYVVSEPLGR YDIALVQEVR |
50–55 kDa | 52 kDa | 55 kDa | Yes | Yes | YDIALVQEVR | LLDNLNQDAPDTYHYVVSEPLGR |