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. 2015 Jun 11;10(6):e0129485. doi: 10.1371/journal.pone.0129485

Table 3. Mass spectrometry analysis of renal tubular cell lysates size-fractionated by SDS gel electrophoresis.

Gel regions as MW spans that are subjected to mass spectrometry Bands recognized by anti-DNaseI antibody on western blot Presence of DNaseI protein verified by mass spectrometry Common DNaseI peptides identified by mass spectrometry
Nuclear extract Cytoplasmic extract Nuclear extract Cytoplasmic extract Nuclear extract Cytoplasmic extract
38–40 kDa No bands 40 kDa No Yes Not detected LLDNLNQDAPDTYHYVVSEPLGR YDIALVQEVR
50–55 kDa 52 kDa 55 kDa Yes Yes YDIALVQEVR LLDNLNQDAPDTYHYVVSEPLGR