Fig 1. Hyperoxia increases Hsp70 expression.

PAECs were exposed to normoxia (room air, 5% CO₂) or to hyperoxia (95% oxygen, 5% CO₂) for 1 to 24 h or 48 h after which the protein and mRNA levels of Hsp70 were measured. (A) Representative immunoblots of Hsp70. (B) Bar graph show the changes in Hsp70 protein levels quantified by scanning densitometry. (C) Bar graph showing the changes in the mRNA levels of hspA1A, hspA1B and hspA2 quantified by quantitative real-time PCR. Results are expressed as mean ± SE; n = 4. *P<0.05 vs. normoxia.