Fig 1. PiBA proof-of-feasibility and mechanism of action.

A) Results suggesting LAMP product is too short to induce bead aggregation, but still capable of binding to silica surface of beads and inhibiting aggregation by ‘trigger’ DNA. B) Effect of LAMP product inhibiting aggregation in presence of various types of ‘trigger’ DNA. C) Following LAMP, ‘trigger’ DNA is added to sample and exposed to RMF for 5 minutes. Images are analyzed and a Yes/No read-out is given in a matter of minutes.