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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: J Invest Dermatol. 2015 Mar 11;135(7):1801–1809. doi: 10.1038/jid.2015.102

Table 2. Summary of Yeast-two Hybrid Data: Interaction of Profilaggrin N-terminus with Human Annexin II/p36 and Stratifin/14-3-3σ.

Bait Protein (pOBD construct) Prey Protein (pOAD construct) Strength of interaction a
Annexin II, 1-339 Human AB +++
Annexin II, 1-339 Human AB (S100 mutant)b +/-c
Annexin II, 1-339 Human A ++
Annexin II, 1-339 Mouse AB ++
Annexin II, 1-339 Mouse A ++
Annexin II, 1-44 Human AB ++
Annexin II, 1-44 Mouse A ++
Annexin II, 15-339 Human AB -
Annexin II, 15-339 Mouse A -
Stratifin Human AB d ++
Stratifin Human AB (S100 mutant)b ++
Stratifin Human A -
Stratifin Human B +
Stratifin Mouse AB ++
Stratifin Mouse A +
Stratifin Mouse B +
Stratifin Stratifin +/-c
a

+++, robust growth on 10 mM 3-AT, ++, weaker growth (and less colonies) on 10 mM 3-AT; +, weak growth on 10 mM 3-AT.

b

This protein contains mutations in key amino acids involved in calcium binding (see text for details).

c

Very weak but detectable growth on histidine-deficient media containing 10 mM 3-AT.

d

Stratifin exhibited a positive interaction with all human profilaggrin constructs containing varying lengths of the B domain, i.e. PF-AB 120, PF-AB 140, PF-AB 160, PF-AB 218, PF-AB 259 and PF-AB 293. Growth was similar (++) for all bait-prey combinations on histidine-deficient media containing 10 mM 3-AT.