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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: J Invest Dermatol. 2015 Mar 9;135(7):1839–1848. doi: 10.1038/jid.2015.91

Figure 3. c-IAP2 and c-FLIP are up-regulated by TNFα and PLX4720 co-treatment.

Figure 3

a) A375 and M238 cells were treated with −/+ 50 ng/ml TNFα and −/+ 5 µM PLX4720 for 6 or 24 hours before analysis by Western blotting. Normalized c-FLIP/Actin band intensity ratios were shown. b) A375 and M238 cells were treated as in a) and total RNA isolated and qRT-PCR performed on c-IAP2 and c-FLIP genes. Housekeeping genes, actin or EEFA1, were internal controls. Average results from three independent experiments are shown. Error bars represent standard deviation. * p<0.05; ** p<0.01; *** p<0.001. c) A375 and M238 cells were transfected with indicated siRNAs for 72 hours and then treated with −/+ 50 ng/ml TNFα and −/+ 5 µM PLX4720 for additional 24 hours. Cells were lysed for Western blotting analysis on indicated proteins. Normalized c-FLIP/Actin band intensity ratios are indicated.