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. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: Transgenic Res. 2013 Dec 15;23(2):341–349. doi: 10.1007/s11248-013-9773-9

Figure 1. The transgene.

Figure 1

(A). The mAlb-hCRP transgene construct is composed two sequences fragments from the 5′-flanking region of mouse albumin gene, and the human CRP structure gene without promoter as well as the 3′-end of the human CRP gene. Blue dots indicate the locations of androgen receptor binding sites predicted by MAPPER and PROMO software, the pink dots indicate the locations of predicted estrogen receptor binding sites. (B). The confirmation of transgene structure in the genomes of three transgenic lines by two specific PCRs. The first PCR used an upstream primer in the albumin enhancer and a downstream primer in the albumin promoter (primer set a). The second PCR used an upstream primer in the albumin promoter and a downstream primer in the CRP gene (primer set b). Totally 11 transgenic rats from three transgenic lines were examined for re-arrangement after transgene incorporation into the rat genome.