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. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: Nat Neurosci. 2014 Nov 24;18(1):25–35. doi: 10.1038/nn.3887

Figure 7.

Figure 7

Human iSNs display physiological properties of mature sensory neurons. (a) The observed frequency of iSNs types in all cells patched. (b,c) Representative traces from whole-cell patch-clamp recordings showing multiple-spiking (b) and single-spiking (c) type iSNs, with single action potentials shown to the right. (d,f) The input resistance of multiple-spiking (d) and single-spiking (f) iSNs plotted as a function of the injected current. (e,g) The number of action potentials fired at increasing levels of current for multiple-spiking (e) and single- spiking (g) iSNs. (h,i) Time course of inward current during perfusion with 100 nM TTX (gray area) and during wash out (white area). Insets are representative current traces before and during the TTX application. The effect was reversible in both cells. (j) iSNs selectively expressed the TTX-resistant sodium channel SCN10A. Expression of MAP2 and SCN10A in HEFs, BAZ iSNs and iSNs generated by BN1 and BN2 was measured by real-time RT-PCR from two independent experiments. Gene expression was normalized to BN1 iSNs. n.d. signifies undetectable expression of that gene. Error bars represent s.e.m. from three experiments.