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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Exp Eye Res. 2015 May 5;136:29–33. doi: 10.1016/j.exer.2015.05.001

Fig. 2.

Fig. 2

Activation of Rho GTPase regulated contractile activity by elevated IOP in the trabecular meshwork of porcine eyes. A. TM derived from eyes perfused under elevated pressure (50 mm Hg) showed significantly increased levels of activated RhoA (RhoA-GTP form) compared to TM derived from eyes perfused at basal pressure (15 mm Hg). Levels of activated RhoA were normalized to total RhoA protein levels. B. TM tissue derived from eyes perfused at 50 mm Hg showed a significant increase in levels of phosphorylated-MLC, MYPT1, MARCKS and Paxillin compared to TM tissue from eyes perfused at 15 mm Hg. pMLC immunoblots were normalized to the total MLC loading control, while other immunoblot data were normalized to the β-actin loading control. Histograms depict values derived from densitometric analysis of immunoblots. Values represent mean± Standard deviation. n = 3, *p < 0.05.