Fig. 2. Blood EGFP transgene expression was restricted to monocytes.
Flow cytometry analysis of peripheral blood monocytes from our CD68-Grx2a-EGFP transgenic (Tg) and wildtype (Wt) mice. In our CD68-Grx2a-EGFP transgenic mice, EGFP was expressed in 5.5% of total leukocytes. Within the EGFP (+) gate there was a shift in CD115+ expression thereby showing the blood transgene expression was restricted to CD115+ cells, i.e. blood monocytes. Within the EGFP (−) gate cells were CD115+ negative.