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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Atherosclerosis. 2015 Apr 26;241(1):69–78. doi: 10.1016/j.atherosclerosis.2015.04.805

Fig. 3. Transgenic Grx2a-EGFP fusion protein is expressed in peritoneal macrophages.

Fig. 3

(A) Flow cytometric analysis of peritoneal macrophages from wildtype (Wt) and transgenic CD68-Grx2aTg mice (Tg). Macrophages were identified based on F4/80 and CD115, and transgenic cells based on their expression of EGFP. Note 73.9% ± 3.0 of CD115+F4/80+ cells from transgenic mice were EGFP positive (n = 3). (B) Western Blot analysis using an anti-Grx2a antibody of purified peritoneal macrophages isolated from wildtype (Wt) and transgenic CD68-Grx2aTg mice (Tg). The 45 kDa band corresponding to the Grx2a-EGFP fusion protein is denoted with (*).