Table 3.

Effect of HFD-feeding on blood monocyte subset distributions in LDLR^−/− and transgenic Grx2a_Mac^LDLR−/− mice.

Monocyte Subset	Baseline [%]	14 Weeks HFD [%]
Females
Total Monocytes (CD68hi)
Wt	6.13 ± 0.55	11.44 ± 1.11 *
Tg	4.87 ± 0.29	11.56 ± 1.50 *
Inflammatory Monocytes (CD68hi Gr-1hi)
Wt	2.59 ± 0.47	2.91 ± 0.32
Tg	1.87 ± 0.17	2.66 ± 0.38
Patrolling Monocytes (CD68hi Gr-1lo)
Wt	2.13 ± 0.16	5.66 ± 0.58 *
Tg	1.89 ± 0.16	5.71 ± 0.87 *
Males
Total Monocytes (CD68hi)
Wt	5.61 ± 0.48	14.52 ± 0.90 *
Tg	5.91 ± 0.49	11.16 ± 1.06 *
Inflammatory Monocytes (CD68hi Gr-1hi)
Wt	1.76 ± 0.14	3.56 ± 0.19
Tg	2.60 ± 0.39	3.06 ± 0.40
Patrolling Monocytes (CD68hi Gr-1lo)
Wt	2.56 ± 0.24	7.52 ± 0.58 *‡
Tg	1.95 ± 0.15	5.39 ± 0.46 *

Blood was obtained from the tail vein of LDLR^−/− (Wt) and transgenic Grx2a_Mac^LDLR−/− mice (Tg) both prior to switching the mice to a HFD diet and after 14 weeks on a HFD. Red blood cells were lysed, and leukocyte staining and FACS analysis was performed as described under Material and Methods. Results for total monocytes(CD68^hi), inflammatory monocytes (CD68^hi/Gr-1^lo) and patrolling monocytes (CD68^hi/Gr-1^lo) are expressed as percent of total leukocyte counts.

^*: P<0.05, baseline vs. 14 weeks HFD;

^‡: P<0.05, Wt (n= 10) vs. Tg (n= 13).