Skip to main content
. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Bone. 2015 May 9;78:150–164. doi: 10.1016/j.bone.2015.05.007

Figure 3. Comparison of osteoblast markers in intramembranous ossification in Bsp null versus controls.

Figure 3

Osteoblast markers were assayed in regions of (A-H) alveolar and (I-P) mandibular bone formation in WT and Bsp−/− mice at 1 dpn. (A-D, I-L) No differences in early osteoblast marker osterix (OSX) are observed in Bsp null compared WT, with OSX-positive cells covering bone surfaces. Yellow boxes in A and C indicate areas shown under higher magnification in B and D, respectively. Yellow boxes in I and K indicate areas shown under higher magnification in J and L, respectively. (E, G and M, O) Tissue nonspecific alkaline phosphatase (TNAP) is present at bone surfaces in WT and Bsp null bones compared to WT, with some suggestion of increased TNAP at certain locations in Bsp−/− mice versus WT. (F, H and N, P) Osteopontin (OPN) is similarly distributed in bone matrix of alveolar and mandibular bone in WT and Bsp−/− mice. (Q-S) Quantitative PCR on mRNA from 5 dpn WT and Bsp−/− mandibles indicated no significant differences (p > 0.05 for n=4-5 samples) in expression of Sp7 (Osterix), Alpl (TNAP), or Spp1 (OPN).