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. 2015 Jun 11;10(6):e0129992. doi: 10.1371/journal.pone.0129992

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© 2015 Singh et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — To quantify egg viability (A) and fecundity (B) Females were held without access to males post cold shock. In this experiment, females were allowed to interact with males initially. However, after cold shock (or control treatment), females were not allowed to interact with males. We then assayed their egg viability (A) and fecundity (B) 24 hours later. Egg viability and fecundity of females subjected to cold shock and not allowed to interact with males thereafter was significantly lower. We found no significant differences in egg viability or fecundity between FCB (open bars) and FSB (closed bars) in either of the treatments. These results are in contrast to the results presented in Figs 1 and 2 wherein females had access to males post cold shock. Thus, taken together, these results indicate that interaction with males post cold shock is necessary for egg viability and fecundity to improve.