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. 2015 Jun 11;11(6):e1004920. doi: 10.1371/journal.ppat.1004920

Fig 7. Importins α/2β1 and transportin are not involved in VP2 nuclear import.

Fig 7

Transport of VP2-trimer (t) analyzed by confocal microscopy in Digitonine-permeabilized HeLa cells, in combination with competitor markers. The transport phenotype of 200 ng of gradient-purified VP2(t) in the presence of 10 μg of BSA conjugated to peptides of characterized import routes (BSA-NLS and BSA-M9), or of 3.5 μM of NLS peptide alone (NLSp), are shown. Transport factors were provided by rabbit reticulocyte lysates (first five columns) or by purified importins (right columns). Representative results from three experiments are shown. Scale bar 10 μm.