Fig 1. DNA competition binding assay for MvaT binding to cupA1 promoter DNA and PA3900 fragment.

Complexes of MvaT (300 nM) with 1 nM radiolabeled AT-rich cupA1 promoter DNA fragment (upper panel) or a radiolabeled GC-rich PA3900 DNA fragment (lower panel), were pre-formed prior to the addition of excess unlabeled competitor DNA as indicated.