Figure 3. Metabolism-dependent localization of the nitrate reductase OXPHOS complex.
Mean frequency of LCB3635 cells displaying clusters at the cell poles for various metabolic conditions (anaerobic respiration with either nitrate, fumarate or TMAO as terminal electron acceptor, aerobic respiration and anoxic fermenting conditions). Non-fermentable glycerol was used as sole carbon and electron source in all cases with the exception of fermenting conditions where it was replaced for glucose. Polar localization is only observed under anaerobic respiration whatever the terminal electron acceptor used.
Figure 3—figure supplement 1. Comparative analysis of cells displaying clusters at the cell poles vs cells displaying clusters along the cell body upon varying metabolic conditions.
(Gray bars) Mean frequency of LCB3635 cells displaying clusters of the fluorescence signal at the cell poles. (Black bars) Mean frequency of LCB3635 cells displaying clusters of the fluorescence signal along the entire cell body for various metabolic conditions. The conditions tested were: anaerobic respiration with either nitrate, fumarate or TMAO as terminal electron acceptor, aerobic respiration, and anoxic fermenting conditions.