Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 12;10(6):e0129879. doi: 10.1371/journal.pone.0129879

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

PMC Copyright notice

Fig 1 — A) Gating strategy for the identification of hIRA B cells in tonsils. Tonsil-derived cells were obtained from tonsils of individuals undergoing routine tonsillectomy. After processing in the presence of DNAse and collagenase, samples were stained, fixed and analyzed by flow cytometry. Live cells were discriminated using Aqua Live/Dead staining kit, and singlet lymphocytes were selected on the basis of forward (FSC) and side (SSC) scatters. B cells were further identified as CD19⁺CD20⁺ and analyzed for the expression of GM-CSF. B) Quantification of hIRA B cells in tonsils. The number of CD19⁺CD20⁺GM-CSF⁺ cells was normalized on the number of total CD19⁺CD20⁺ lymphocytes after isotype background subtraction. The bar represents the geometric mean.