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. 2015 Jun 12;10(6):e0129879. doi: 10.1371/journal.pone.0129879

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Fig 4 — Conventional CD19⁺CD20⁺GM-CSF^-(GM-CSF^neg) and hIRA CD19⁺CD20⁺GM-CSF⁺(GM-CSF^pos) B cells in cell suspensions from tonsils were analyzed by flow cytometry. A) Expression of CD5, CD43, CD11c and IgD in GM-CSF^neg versus GM-CSF^pos cells. The graphs show the geometric mean of fluorescence intensity (GeoMFI) for each marker after isotype background subtraction. Means and standard deviations are shown. P value was calculated using the non-parametric Mann–Whitney test (n = 4). B) Ig subclasses were analyzed within the hIRA B cell population. The plots are representative of n = 4.